Selected article for: "reference genome and shotgun sequencing"
Author: Corey T Watson; Karyn Meltz Steinberg; Tina A Graves-Lindsay; Rene L Warren; Maika Malig; Jacqueline E Schein; Richard K Wilson; Rob Holt; Evan Eichler; Felix Breden
Title: Sequencing of the human IG light chain loci from a hydatidiform mole BAC library reveals locus-specific signatures of genetic diversity
  • Document date: 2014_7_3
    • ID: 62gfisc6_35
    Snippet: BAC-end reads from the CHORI-17 hydatidiform mole BAC library mapping to the GRCh37 reference genome were used to identify and select clones within the two light chain regions. The IGL and IGK genes are located at distinct loci in the genome, 22q11.2 and 2p11.2, respectively 2,13,17,49-51 . Nine clones mapping to the IGL region, and eight clones mapping to IGK were picked for complete sequencing. As described in Watson et al 7 clones were shotgun.....
    Document: BAC-end reads from the CHORI-17 hydatidiform mole BAC library mapping to the GRCh37 reference genome were used to identify and select clones within the two light chain regions. The IGL and IGK genes are located at distinct loci in the genome, 22q11.2 and 2p11.2, respectively 2,13,17,49-51 . Nine clones mapping to the IGL region, and eight clones mapping to IGK were picked for complete sequencing. As described in Watson et al 7 clones were shotgun sequenced using high quality capillary-based Sanger sequencing and assemblies were constructed and finished on a per clone basis. Fully assembled overlapping BAC clones were then used to create contiguous assemblies spanning the two IG light chain regions using SeqMan Pro (DNA Star, Lasergene, Wisconsin, USA).

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