Selected article for: "chain RT PCR reaction and collection tube"

Author: Lunwen Wang; Xun Li; Hui Chen; Shaonan Yan; Yan Li; Dong Li; Zuojiong Gong
Title: SARS-CoV-2 infection does not significantly cause acute renal injury: an analysis of 116 hospitalized patients with COVID-19 in a single hospital, Wuhan, China
  • Document date: 2020_2_23
  • ID: 368pz09b_11
    Snippet: All COVID-19 patients enrolled in this study were laboratory-confirmed cases, which were identified with nucleic acid detection of SARS-CoV-2 from a throat swab samples using reverse transcription-polymerase chain reaction (RT-PCR). The criteria for the confirmed-diagnosis of SARS-CoV-2 was that at least one gene site was amplified to be positive for nucleocapsid protein (NP) gene and open reading frame (ORF) 1ab gene. In brief, the throat swab w.....
    Document: All COVID-19 patients enrolled in this study were laboratory-confirmed cases, which were identified with nucleic acid detection of SARS-CoV-2 from a throat swab samples using reverse transcription-polymerase chain reaction (RT-PCR). The criteria for the confirmed-diagnosis of SARS-CoV-2 was that at least one gene site was amplified to be positive for nucleocapsid protein (NP) gene and open reading frame (ORF) 1ab gene. In brief, the throat swab was put into a collection tube containing 150μl viral preservation solution, and the total RNA was extracted within 2h with the respiratory sample RNA separation Kit (Zhongzhi, Wuhan). The suspension All rights reserved. No reuse allowed without permission.

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