Author: Wang, Huanan; Liu, Xiangnan; Zeng, Fanwen; Zhang, Tongyuan; Lian, Yuexiao; Wu, Miaoli; Xiao, Li; Zhu, Yujun; Zhang, Yu; Chen, Meili; Huang, Ren; Luo, Manlin; Cong, Feng; Guo, Pengju
Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3 Cord-id: v06dpgoi Document date: 2019_8_23
ID: v06dpgoi
Snippet: BACKGROUND: Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland. RESULTS: A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 × 10(1) copies/μL with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth dise
Document: BACKGROUND: Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland. RESULTS: A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 × 10(1) copies/μL with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203). CONCLUSIONS: The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3.
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