Author: Ke Wang; Wei Chen; Yu-Sen Zhou; Jian-Qi Lian; Zheng Zhang; Peng Du; Li Gong; Yang Zhang; Hong-Yong Cui; Jie-Jie Geng; Bin Wang; Xiu-Xuan Sun; Chun-Fu Wang; Xu Yang; Peng Lin; Yong-Qiang Deng; Ding Wei; Xiang-Min Yang; Yu-Meng Zhu; Kui Zhang; Zhao-Hui Zheng; Jin-Lin Miao; Ting Guo; Ying Shi; Jun Zhang; Ling Fu; Qing-Yi Wang; Huijie Bian; Ping Zhu; Zhi-Nan Chen
Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein Document date: 2020_3_14
ID: 5hpbjkft_25
Snippet: Co-IP assay was performed using Pierce ® Co-Immunoprecipitation Kit (26149, Thermo Fisher Scientific, USA) according to the manufacturer's protocol. Anti-CD147 antibody (HAb18, produced by our laboratory) and anti-SARS-CoV-2 Spike antibody (40150-R007, Sino Biological, China) were used for antibody immobilization for Co-IP. The eluted proteins were detected by western blot. After boiling for 5-10 minutes, the eluted proteins were loaded to 12% S.....
Document: Co-IP assay was performed using Pierce ® Co-Immunoprecipitation Kit (26149, Thermo Fisher Scientific, USA) according to the manufacturer's protocol. Anti-CD147 antibody (HAb18, produced by our laboratory) and anti-SARS-CoV-2 Spike antibody (40150-R007, Sino Biological, China) were used for antibody immobilization for Co-IP. The eluted proteins were detected by western blot. After boiling for 5-10 minutes, the eluted proteins were loaded to 12% SDS-PAGE gel and then transferred to PVDF author/funder. All rights reserved. No reuse allowed without permission.
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