Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_15
Snippet: Furthermore, comparable levels of PARP2 were observed between F20, COS-7 and MCF-311 7 cells (data not shown). F20 cells were cultured in CM until 90% confluence and then 312 pretreated with 250µM leupeptin (LEU), 30µM pepstatin A methyl ester (PME), 30µM 313 calpeptin (CAL), or 50µgml -1 α-2-macroglobulin (α2-M). After 5hr, the medium was 314 replaced with SF medium containing the corresponding protease inhibitor. To inactivate a 315 broad.....
Document: Furthermore, comparable levels of PARP2 were observed between F20, COS-7 and MCF-311 7 cells (data not shown). F20 cells were cultured in CM until 90% confluence and then 312 pretreated with 250µM leupeptin (LEU), 30µM pepstatin A methyl ester (PME), 30µM 313 calpeptin (CAL), or 50µgml -1 α-2-macroglobulin (α2-M). After 5hr, the medium was 314 replaced with SF medium containing the corresponding protease inhibitor. To inactivate a 315 broad spectrum of endo-and exopeptidases, cells were treated with the 1x HALT Protease 316
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