Selected article for: "design process and probe design process"
Author: Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee
Title: Sensitive one-step isothermal detection of pathogen-derived RNAs
  • Document date: 2020_3_9
    • ID: 1uqjmeic_15
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is Since the one-step and one-pot isothermal reaction condition was established, we then 157 assessed the sensitivity and turnaround time of SENSR. We evaluated the sensitivity by 158 measuring fluorescence from one-step reactions containing the mecA probe pair and synthetic 159 mecA RNA in the range of 0.1 aM to 220 nM (Fig. 3a) . Notably, the detection limit was as 160 With th.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is Since the one-step and one-pot isothermal reaction condition was established, we then 157 assessed the sensitivity and turnaround time of SENSR. We evaluated the sensitivity by 158 measuring fluorescence from one-step reactions containing the mecA probe pair and synthetic 159 mecA RNA in the range of 0.1 aM to 220 nM (Fig. 3a) . Notably, the detection limit was as 160 With the fast and sensitive RNA detection using SENSR, we next attempted to reconfigure 174 this platform for the detection of RNA markers from various pathogens. Target RNA 175 sequences for SENSR are specified by only two hybridization regions (UHS and DHS) of 176 probes, which makes the probe design process fast and straightforward without many 177 All rights reserved. No reuse allowed without permission. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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