Author: Laura E Lamb; Sarah N Bartolone; Elijah Ward; Michael B Chancellor
Title: Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification Document date: 2020_2_24
ID: 6kpgt70s_4
Snippet: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one-step nucleic acid amplification method based on PCR technology that has been used to diagnose infectious diseases. 2 RT-LAMP has several advantages including that it has high specificity and sensitivity, can be done in less than an hour, can work at various pH and temperature ranges which is advantageous for clinical samples, 3 and that the reagents are relatively low.....
Document: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one-step nucleic acid amplification method based on PCR technology that has been used to diagnose infectious diseases. 2 RT-LAMP has several advantages including that it has high specificity and sensitivity, can be done in less than an hour, can work at various pH and temperature ranges which is advantageous for clinical samples, 3 and that the reagents are relatively low cost and can be stable at room temperature. We have previously used this method to detect zika virus in clinical serum and urine samples as well as mosquitos. 4, 5 This study describes a RT-LAMP methodology that can detect COVID-19 in simulated patient samples in under 30 minutes. The test could be used at the point-of-care by field and local personnel for the rapid diagnosis of individuals for optimal treatment, isolation, and rapid contact tracking as well as the investigation of outbreaks of unknown respiratory diseases.
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