Author: Shi, Xibao; Wang, Li; Li, Xuewu; Zhang, Gaiping; Guo, Junqing; Zhao, Dong; Chai, Shujun; Deng, Ruiguang
Title: Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-β induction Cord-id: vdannfrd Document date: 2011_4_9
ID: vdannfrd
Snippet: Previous studies have shown that PRRSV nsp11, which was an endoribonuclease, was an interferon antagonist, however, the mechanism that nsp11 inhibited IFN-β production was unclear. To explore whether the endoribonuclease was required for nsp11 to disrupt the IFN-β production, substitutions of the presumed catalytic histidine and lysine residues of nsp11 were introduced into plasmid pcDNA 3.1-FLAG. The results showed that mutation that inactivated endoribonuclease made nsp11 lose its ability to
Document: Previous studies have shown that PRRSV nsp11, which was an endoribonuclease, was an interferon antagonist, however, the mechanism that nsp11 inhibited IFN-β production was unclear. To explore whether the endoribonuclease was required for nsp11 to disrupt the IFN-β production, substitutions of the presumed catalytic histidine and lysine residues of nsp11 were introduced into plasmid pcDNA 3.1-FLAG. The results showed that mutation that inactivated endoribonuclease made nsp11 lose its ability to inhibit Poly(I:C)-induced IFN-β promoter activity. In conclusion, our present work indicated that the endoribonuclease activity of nsp11 was essential for nsp11 to inhibit the IFN-β induction.
Search related documents:
Co phrase search for related documents- acid inducible and active site: 1
- acid inducible and adaptor molecule: 1, 2, 3
- acid inducible and luciferase reporter: 1, 2
- acid inducible gene and activation trigger: 1, 2, 3, 4, 5, 6
- acid inducible gene and active site: 1
- acid inducible gene and adaptor molecule: 1, 2, 3
- acid inducible gene and luciferase reporter: 1, 2
- acid inducible gene retinoic and activation trigger: 1, 2, 3, 4, 5, 6
- acid inducible gene retinoic and active site: 1
- acid inducible gene retinoic and adaptor molecule: 1, 2, 3
- acid inducible gene retinoic and luciferase reporter: 1, 2
- activation trigger and adaptor molecule: 1
- active site and luciferase activity: 1
Co phrase search for related documents, hyperlinks ordered by date