Coronavirus Antigens: Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens (Adv. Biology 4/2021)

Kim, Chonsaeng; Jeong, You Kyeong; Yu, Jihyeon; Shin, Hye Jin; Ku, Keun Bon; Cha, Hyung Jin; Han, Jun Hee; Hong, Sung‐Ah; Kim, Bum‐Tae; Kim, Seong‐Jun; Woo, Jae‐Sung; Bae, Sangsu

The production of biomolecules that require human‐specific lipid environments is extremely useful for basic research and medical applications. In article number 2000154, Seong‐Jun Kim, Jae‐Sung Woo, Sangsu Bae, and co‐workers integrate multiple proteins or virus antigens into defined transcriptional hotspots in the human genome via a homology‐independent targeted insertion method using CRISPR nucleases. This system is similar to a production pipeline of biomolecules in a factory controlled by CRISPR. [Image: see text]