Author: Parra-Sánchez, Héctor; Bustamante-Córdova, Lorena; Reséndiz, Mónica; Mata-Haro, Verónica; Pinelli-Saavedra, Araceli; Hernández, Jesús
Title: Analysis of Swine Conventional Dendritic Cells, DEC205(+)CD172a(+/−)CADM1(+), from Blood and Spleen in Response to PRRSV and PEDV Cord-id: zn9nagv9 Document date: 2019_10_31
ID: zn9nagv9
Snippet: Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive and respiratory syndrome virus (PRRSV) but respond to infection via cytokine production, indicating a possible role in initiation/regulation of the immune response against PRRSV. In this work, we evaluated the responses of splenic and blood cDCs, with DEC205(+)CADM1(+)CD172a(+/−) phenotype, as well as those of CD163(+) cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both populations were incubated in
Document: Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive and respiratory syndrome virus (PRRSV) but respond to infection via cytokine production, indicating a possible role in initiation/regulation of the immune response against PRRSV. In this work, we evaluated the responses of splenic and blood cDCs, with DEC205(+)CADM1(+)CD172a(+/−) phenotype, as well as those of CD163(+) cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both populations were incubated in the presence of PRRSV or PEDV with and without naïve CD3(+) T cells, and cytokine responses were evaluated by qPCR and ELISA. Our results showed that cDCs, but not CD163(+) cells, produced IL-12 in response to PRRSV. PEDV did not induce IL-12 production. Cocultures of cDCs and autologous naïve CD3(+) cells resulted in decreased IL-12 production and low expression of IFN-γ transcripts in response to PRRSV. Interestingly, cDCs increased the proliferation of naïve T cells in the presence of PRRSV compared with that achieved with monocytes and peripheral blood mononuclear cells (PBMCs). Cocultures of CD163(+) cells induced IL-10 and IL-4 expression in the presence of PRRSV and PEDV, respectively. In conclusion, cDCs can selectively produce IL-12 in response to PRRSV but poorly participate in the activation of naïve T cells.
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