Author: Yinhua Zhang; Nelson Odiwuor; Jin Xiong; Luo Sun; Raphael Ohuru Nyaruaba; Hongping Wei; Nathan A Tanner
Title: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP Document date: 2020_2_29
ID: cv3qgno3_3
Snippet: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.02.26.20028373 doi: medRxiv preprint blue (8) . We recently developed a method for visual detection of LAMP amplification (9) using pH-sensitive dyes to exploit the change of pH that resulting from proton accumulation due to dNTP incorporation. This method has been used in a large scale field survey of Wolbachia-containing mosquitos (10), Gr.....
Document: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.02.26.20028373 doi: medRxiv preprint blue (8) . We recently developed a method for visual detection of LAMP amplification (9) using pH-sensitive dyes to exploit the change of pH that resulting from proton accumulation due to dNTP incorporation. This method has been used in a large scale field survey of Wolbachia-containing mosquitos (10), Grapevine red blotch virus without DNA extraction (11) , testing urine samples for Zika virus (12) and even amplification detection on the International Space Station (13) . The breadth of application highlights the applicability of visual detection methods to provide an advantage in simplicity and portability for enabling new, rapid diagnostics.
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