Selected article for: "detection procedure and rna extraction"
Author: Yinhua Zhang; Nelson Odiwuor; Jin Xiong; Luo Sun; Raphael Ohuru Nyaruaba; Hongping Wei; Nathan A Tanner
Title: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
  • Document date: 2020_2_29
    • ID: cv3qgno3_4
    Snippet: This study describes testing and validation of 5 sets LAMP primers targeting two fragments of the SARS-CoV-2 genome using short (~300bp) RNA fragments made with in vitro transcription and RNA samples from patients. We also demonstrate compatibility with simple approaches to RNA purification to simplify the detection procedure and avoid complex RNA extraction, and with clinical swab samples taken from COVID-19 patients. Our aim is to share this in.....
    Document: This study describes testing and validation of 5 sets LAMP primers targeting two fragments of the SARS-CoV-2 genome using short (~300bp) RNA fragments made with in vitro transcription and RNA samples from patients. We also demonstrate compatibility with simple approaches to RNA purification to simplify the detection procedure and avoid complex RNA extraction, and with clinical swab samples taken from COVID-19 patients. Our aim is to share this information in order to help develop a reliable and easy method to detect this viral RNA outside of sophisticated diagnostic laboratories and expand the toolbox of molecular tests used to combat and surveil this growing public health threat.

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