Selected article for: "mRNA level and qrt pcr"
Author: Nila Roy Choudhury; Gregory Heikel; Maryia Trubitsyna; Peter Kubik; Jakub Stanislaw Nowak; Shaun Webb; Sander Granneman; Christos Spanos; Juri Rappsilber; Alfredo Castello; Gracjan Michlewski
Title: RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination
  • Document date: 2017_10_9
    • ID: ifla4aix_29
    Snippet: To gain further insights into TRIM25's role in RNA metabolism we have used qRT-PCR to analyze mRNA levels of selected targets (the same ones as assayed by RIP in Fig. 4f -g) and ZAP mRNA in the wild type and TRIM25 KO cells. The majority of analyzed transcripts did not display substantial difference between wild type and KO cells ( Figure S7a Figure S7c -e). This suggests that the steady-state ZAP mRNA level change seen in the TRIM25 KO cells is .....
    Document: To gain further insights into TRIM25's role in RNA metabolism we have used qRT-PCR to analyze mRNA levels of selected targets (the same ones as assayed by RIP in Fig. 4f -g) and ZAP mRNA in the wild type and TRIM25 KO cells. The majority of analyzed transcripts did not display substantial difference between wild type and KO cells ( Figure S7a Figure S7c -e). This suggests that the steady-state ZAP mRNA level change seen in the TRIM25 KO cells is due to transcriptional control. This is not surprising as a recent study revealed that TRIM25 is a potent transcriptional regulator (Walsh et al., 2017) . Further indepth and high throughput experiments will be needed to fully understand the potential role of TRIM25 in RNA metabolism.

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