Selected article for: "recent study and wild type"
Author: Nila Roy Choudhury; Gregory Heikel; Maryia Trubitsyna; Peter Kubik; Jakub Stanislaw Nowak; Shaun Webb; Sander Granneman; Christos Spanos; Juri Rappsilber; Alfredo Castello; Gracjan Michlewski
Title: RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination
  • Document date: 2017_10_9
    • ID: ifla4aix_29
    Snippet: To gain further insights into TRIM25's role in RNA metabolism we have used qRT-PCR to analyze mRNA levels of selected targets (the same ones as assayed by RIP in Fig. 4f -g) and ZAP mRNA in the wild type and TRIM25 KO cells. The majority of analyzed transcripts did not display substantial difference between wild type and KO cells ( Figure S7a Figure S7c -e). This suggests that the steady-state ZAP mRNA level change seen in the TRIM25 KO cells is .....
    Document: To gain further insights into TRIM25's role in RNA metabolism we have used qRT-PCR to analyze mRNA levels of selected targets (the same ones as assayed by RIP in Fig. 4f -g) and ZAP mRNA in the wild type and TRIM25 KO cells. The majority of analyzed transcripts did not display substantial difference between wild type and KO cells ( Figure S7a Figure S7c -e). This suggests that the steady-state ZAP mRNA level change seen in the TRIM25 KO cells is due to transcriptional control. This is not surprising as a recent study revealed that TRIM25 is a potent transcriptional regulator (Walsh et al., 2017) . Further indepth and high throughput experiments will be needed to fully understand the potential role of TRIM25 in RNA metabolism.

    Search related documents:
    Co phrase search for related documents
    • KO cell and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • KO cell wild type and wild type: 1, 2
    • level change and mRNA level: 1, 2, 3, 4, 5, 6
    • level change and potential role: 1, 2, 3, 4
    • level change and recent study: 1, 2
    • level change and steady state: 1, 2
    • level change and substantial difference: 1, 2
    • mRNA level and potential role: 1, 2, 3, 4
    • mRNA level and qrt PCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • mRNA level and recent study: 1, 2, 3
    • mRNA level and RIP assay: 1
    • mRNA level and steady state: 1, 2, 3
    • mRNA level and transcriptional control: 1, 2, 3
    • mRNA level and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • potential role and qrt PCR: 1, 2, 3, 4, 5, 6
    • potential role and recent study: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • potential role and RNA metabolism: 1, 2, 3
    • potential role and RNA metabolism role: 1
    • potential role and steady state: 1, 2, 3, 4