Author: Nila Roy Choudhury; Gregory Heikel; Maryia Trubitsyna; Peter Kubik; Jakub Stanislaw Nowak; Shaun Webb; Sander Granneman; Christos Spanos; Juri Rappsilber; Alfredo Castello; Gracjan Michlewski
Title: RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination Document date: 2017_10_9
ID: ifla4aix_86
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https: //doi.org/10.1101/200410 doi: bioRxiv preprint Levels of RNAs of interest were determined by qRT-PCR using SuperScrip III Platinum SYBR Green One-Step qRT-PCR Kit (ThermoFisher). 200ng RNA was loaded per well for RNAs of interest and these levels were normali.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https: //doi.org/10.1101/200410 doi: bioRxiv preprint Levels of RNAs of interest were determined by qRT-PCR using SuperScrip III Platinum SYBR Green One-Step qRT-PCR Kit (ThermoFisher). 200ng RNA was loaded per well for RNAs of interest and these levels were normalised to 18S Ribosomal RNA for which 1ng total RNA was loaded per well (1 in 200 dilution).
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