Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_16
Snippet: Inhibitor Cocktail, which contains AEBSF-HCl, aprotinin, bestatin, E-64, leupeptin, 317 pepstatin A and EDTA. After a 15-minute incubation, cell lysates were collected and 318 processed for immunoblot analysis with the Yucatan PARP2 antibody. Results showed that 319 the inhibitors, either alone or in a cocktail (HALT), were not effective in preventing the 320 disappearance of the PARP2 signal (Fig. 3B ). This led us to conclude that under serum 3.....
Document: Inhibitor Cocktail, which contains AEBSF-HCl, aprotinin, bestatin, E-64, leupeptin, 317 pepstatin A and EDTA. After a 15-minute incubation, cell lysates were collected and 318 processed for immunoblot analysis with the Yucatan PARP2 antibody. Results showed that 319 the inhibitors, either alone or in a cocktail (HALT), were not effective in preventing the 320 disappearance of the PARP2 signal (Fig. 3B ). This led us to conclude that under serum 321 deprivation, PARP2 levels were not affected by an amino, serine, cysteine, metallo-and 322 aspartic acid protease. After exhausting a broad spectrum of candidate proteases thought 323 to be responsible for degrading PARP2, our attention turned to the ubiquitin-proteasome 324 system (UPS). 325 All rights reserved. No reuse allowed without permission.
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