Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_3
Snippet: The cells were incubated for 7hr, and then the medium replaced with CM containing 128 50µgml -1 CHX. The cells were cultured for 1hr and then lysed in Laemmli sample buffer. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/483289 doi: bioRxiv preprint using primers specific to human PARP2 (forward 5'-GAATCTGTGAAGGCCTTGCTG-140 3' and reverse 5'-TTCCCACCCAGTTACTCATCC-3'). PCR prod.....
Document: The cells were incubated for 7hr, and then the medium replaced with CM containing 128 50µgml -1 CHX. The cells were cultured for 1hr and then lysed in Laemmli sample buffer. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/483289 doi: bioRxiv preprint using primers specific to human PARP2 (forward 5'-GAATCTGTGAAGGCCTTGCTG-140 3' and reverse 5'-TTCCCACCCAGTTACTCATCC-3'). PCR products were resolved on 141 1% agarose gels and images captured with a FluorChem IS-8900 Imager ( (Fig. 1) . 231
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