On-site environmental DNA detection of species using ultra-rapid mobile PCR.

Doi, Hideyuki; Watanabe, Takeshi; Nishizawa, Naofumi; Saito, Tatsuya; Nagata, Hisao; Kameda, Yuichi; Maki, Nobutaka; Ikeda, Kousuke; Fukuzawa, Takashi

Molecular methods, including environmental DNA (eDNA) methods, provide essential information for biological and conservation sciences. Molecular measurements are often performed in the laboratory, which limits their scope, especially for rapid on-site analysis. eDNA methods for species detection provide essential information for the management and conservation of species and communities in various environments. We developed an innovative novel method for on-site eDNA measurements using an ultra-rapid mobile PCR platform. We tested the ability of our method to detect the distribution of silver carp, Hypophthalmichthys molitrix in rivers and lakes. Our method reduced the measurement time to 30 min and provided high detectability of aquatic organisms compared to the national observation surveys using multiple fishing nets and laboratory extraction/detection using a benchtop qPCR platform. Our on-site eDNA method can be immediately applied to various taxa and environments.