Selected article for: "dose dependent manner and protein synthesis"
Author: Yang, Jun-Li; Dhodary, Basanta; Quy Ha, Thi Kim; Kim, Jinwoong; Kim, Eunhee; Oh, Won Keun
Title: Three new coumarins from Saposhnikovia divaricata and their porcine epidemic diarrhea virus (PEDV) inhibitory activity
  • Cord-id: w2oucppa
  • Document date: 2015_7_8
    • ID: w2oucppa
    Snippet: Swine based agro-industries throughout the world are in big threat of new PEDV infection due to lack of efficient prophylactic defenses as well as dependable curing agents. Bioactivity-guided fractionation of a methanol soluble extract from radix of Saposhnikovia divaricata led to the isolation of three new (1–3) together with 10 known coumarins (4–13). The structures of new isolates (1–3) were established by extensive spectroscopic analysis and their absolute configurations were assigned
    Document: Swine based agro-industries throughout the world are in big threat of new PEDV infection due to lack of efficient prophylactic defenses as well as dependable curing agents. Bioactivity-guided fractionation of a methanol soluble extract from radix of Saposhnikovia divaricata led to the isolation of three new (1–3) together with 10 known coumarins (4–13). The structures of new isolates (1–3) were established by extensive spectroscopic analysis and their absolute configurations were assigned based on ECD spectra calculation and analysis. Among all isolates, compound 5 revealed strongest inhibitory effect on PEDV replication. Quantitative real-time PCR data showed inhibitory effect of 5 on genes responsible for synthesis of PEDV vital structural proteins (GP6 nucleocapsid, GP2 spike, and GP5 membrane) in a dose-dependent manner. Also, compound 5 demonstrated the inhibitory effect on PEDV GP6 nucleocapsid and GP2 spike protein synthesis as analyzed by western blotting. This study represents a new class of chemical entities for developing anti-PEDV agents.

    Search related documents:
    Co phrase search for related documents
    • actin antibody and lysis buffer: 1, 2, 3, 4, 5, 6
    • activity relationship and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • activity relationship and adjuvant arthritis: 1
    • acute respiratory syndrome and lysis buffer: 1, 2, 3