Single Nucleus Multiomic Profiling Reveals Age-Dynamic Regulation of Host Genes Associated with SARS-CoV-2 Infection

Allen Wang; Joshua A Chiou; Olivier B Poirion; Justin Buchanan; Michael J Valdez; Jamie M Verheyden; Xiaomeng Hou; Minzhe Guo; Jacklyn M Newsome; Parul Kudtarkar; Dina A Faddah; Kai Zhang; Randee E Young; Justinn Barr; Ravi Misra; Heidie Huyck; Lisa Rogers; Cory Poole; Jeffery A Whitsett; Gloria Pryhuber; Yan Xu; Kyle J Gaulton; Sebastian Preissl; Xin Sun

We detected on average 1,662 gene/nuclei for 30wk GA , 1,394 for 3yo and 1,260 for 30yo 181 ( Figure S1C ). Libraries were sequenced to comparable saturation (58.4 % for 30wk GA , 182 51.6 % for 3yo and 55.0 % for 30yo; Fig. S1D ). 183 each dataset, nuclei with >1,000 uniquely mapped sequencing reads were included in the 186 analysis (Fig. S1E) . The average number of nuclei that passed this threshold per age 187 group was 8,691 for 30wk GA , 7,877 for 3yo and 8,034 for 30yo (Fig. S1F ). The average 188 number of reads per nucleus was 6,399 for 30wk GA , 7,199 for 3yo and 8,362 for 30yo 189 (Fig. S1G ). The fraction of reads in peaks (FRiP) on average per data set was 52.8 % for 190 30wk GA , 54.4 % for 3yo and 45.6 % for 30yo (Fig. S1H) . These values indicate 191 consistently high signal to noise ratios for all libraries. an unclassified cluster, and enucleated erythrocytes because the latter were only 204 detected in a single neonatal sample, consistent with immaturity ( Figure 1A) . 205