Author: Bing-peng Lin; Mei Zhong; Hong-bin Gao; Kai-bin Wu; Ming-xiao Liu; Chang Liu; Xin-hong Wang; Jian-ming Chen; Learn-han Lee; Cui-ling Qi; Lin-hu Ge; Li-jing Wang
Title: Significant expression of FURIN and ACE2 on oral epithelial cells may facilitate the efficiency of 2019-nCov entry Document date: 2020_4_18
ID: 0vqzlurx_17
Snippet: Subsequently, they were dried and incubated anti-ACE2 (cat. no. ab15348 , Abcam, Cambridge, CB, UK) antibody overnight at 4 °C. The next day, the HRP-conjugated secondary antibody was added to the sections. Peroxidase activity was developed by using DAB for 8 min and counterstained with hematoxylin. The expression levels of ACE2 were evaluated by two experimenters. A qualified pathologist analyzed the staining for structures positive for ACE2. T.....
Document: Subsequently, they were dried and incubated anti-ACE2 (cat. no. ab15348 , Abcam, Cambridge, CB, UK) antibody overnight at 4 °C. The next day, the HRP-conjugated secondary antibody was added to the sections. Peroxidase activity was developed by using DAB for 8 min and counterstained with hematoxylin. The expression levels of ACE2 were evaluated by two experimenters. A qualified pathologist analyzed the staining for structures positive for ACE2. The stain intensity of ACE2 and FURIN was semiquantitatively graded according to the previous methods [22] .
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