Author: Guangchun Han; Ansam Sinjab; Warapen Treekitkarnmongkol; Patrick Brennan; Kieko Hara; Kyle Chang; Elena Bogatenkova; Beatriz Sanchez-Espiridion; Carmen Behrens; Boning Gao; Luc Girard; Jianjun Zhang; Boris Sepesi; Tina Cascone; Lauren Byers; Don L. Gibbons; Jichao Chen; Seyed Javad Moghaddam; Edwin J. Ostrin; Junya Fujimoto; Jerry Shay; John V. Heymach; John D. Minna; Steven Dubinett; Paul A. Scheet; Ignacio I. Wistuba; Edward Hill; Shannon Telesco; Christopher Stevenson; Avrum E. Spira; Linghua Wang; Humam Kadara
Title: Single-cell analysis of human lung epithelia reveals concomitant expression of the SARS-CoV-2 receptor ACE2 with multiple virus receptors and scavengers in alveolar type II cells Document date: 2020_4_17
ID: j3vruni3_8
Snippet: Fresh tissues were collected in RPMI medium supplemented with 2% fetal bovine serum (FBS) and maintained on ice for immediate processing. Tissues were placed in a cell culture dish containing Hank's balanced salt solution (HBSS) on ice and extra-pulmonary airways and connective tissue was removed with scissors. Samples were transferred to a new dish on ice and minced into tiny pieces (approximately 1mm 3 ) followed by enzymatic digestion using a .....
Document: Fresh tissues were collected in RPMI medium supplemented with 2% fetal bovine serum (FBS) and maintained on ice for immediate processing. Tissues were placed in a cell culture dish containing Hank's balanced salt solution (HBSS) on ice and extra-pulmonary airways and connective tissue was removed with scissors. Samples were transferred to a new dish on ice and minced into tiny pieces (approximately 1mm 3 ) followed by enzymatic digestion using a cocktail containing Collagenase A (10103578001, Sigma Aldrich), Collagenase IV (NC9836075, Thermo Fisher Scientific), DNase I, (11284932001, Sigma Aldrich), Dispase II (4942078001, Sigma Aldrich), Elastase 1 (NC9301601, Thermo Fisher Scientific) and Pronase (10165921001, Sigma Aldrich). Samples were incubated in a 37 °C oven for 10 minutes with gentle rotation and then incubated for an additional 10 minutes.
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