Author: Aaron S. Mendez; Carolin Vogt; Jens Bohne; Britt A. Glaunsinger
Title: Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX Document date: 2018_5_13
ID: 298cbr1x_6
Snippet: To analyze RNA substrate selectivity using our in vitro assay, we first compared SOX degradation of LIMD1 54 to a 51-nucleotide sequence of the mRNA encoding GFP (GFP 51). We have previously shown that GFP mRNA is cleaved by SOX in cells, and that GFP 51 is the minimal sequence required to elicit cleavage (15, 16) . The cleavage sites for LIMD1 54 and GFP 51 are predicted to occur in an open loop region ( Fig. 2A , red arrow). Upon direct compari.....
Document: To analyze RNA substrate selectivity using our in vitro assay, we first compared SOX degradation of LIMD1 54 to a 51-nucleotide sequence of the mRNA encoding GFP (GFP 51). We have previously shown that GFP mRNA is cleaved by SOX in cells, and that GFP 51 is the minimal sequence required to elicit cleavage (15, 16) . The cleavage sites for LIMD1 54 and GFP 51 are predicted to occur in an open loop region ( Fig. 2A , red arrow). Upon direct comparison of these two RNAs, we observed a 5-fold increase in the catalytic efficiency of SOX for the LIMD1 54 substrate compared to GFP 51 (Fig. 2B) .
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