Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_2
Snippet: To investigate how PARP2 is regulated, a survey of several cell lines showed robust 73 7 COS-7, MCF-7, HeLa, NIH3T3, MEF F20 and IMCD cells were maintained in 117 Dulbecco's modified Eagle's medium (DMEM)/F-12 or DMEM supplemented with 10% 118 FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in 5% CO2 at 37ºC. Cells were 119 treated with different protease inhibitors at the concentration and duration as indicated in 120 the figures. Cells.....
Document: To investigate how PARP2 is regulated, a survey of several cell lines showed robust 73 7 COS-7, MCF-7, HeLa, NIH3T3, MEF F20 and IMCD cells were maintained in 117 Dulbecco's modified Eagle's medium (DMEM)/F-12 or DMEM supplemented with 10% 118 FBS, 100 units/ml penicillin and 100 mg/ml streptomycin in 5% CO2 at 37ºC. Cells were 119 treated with different protease inhibitors at the concentration and duration as indicated in 120 the figures. Cells were subject to serum starvation or maintained in medium containing 121 different concentrations of sera where stated. Transfections were carried out using X-122 tremeGENE 9 transfection reagent as per manufacturer's recommendation. To test if the 123 loss of the PARP2 signal on blots under serum starvation conditions was due to 124 sequestration rather than degradation, MCF-7 cells were cultured to approximately 90% 125 confluence in complete medium (CM) and then this was removed and replaced with serum-126 free (SF) medium containing 50µgml -1 cycloheximide (CHX) to inhibit protein synthesis. 127
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