Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_8
Snippet: Since results indicated that PARP2 might be a short-lived protein when cells were 262 cultured in SF medium, an in silico analysis was done to identify PEST sequences 263 (mobyle.pasteur.fr), which are responsible for the rapid turnover of many short-lived 264 proteins (Belizario et al. 2008). Although no putative sites were identified in PARP2, COS-265 7 cells were cultured in CM and protein turnover examined when translation was blocked 266 usi.....
Document: Since results indicated that PARP2 might be a short-lived protein when cells were 262 cultured in SF medium, an in silico analysis was done to identify PEST sequences 263 (mobyle.pasteur.fr), which are responsible for the rapid turnover of many short-lived 264 proteins (Belizario et al. 2008). Although no putative sites were identified in PARP2, COS-265 7 cells were cultured in CM and protein turnover examined when translation was blocked 266 using cycloheximide (CHX). Cells were cultured in the presence of CHX (50µgml -1 ) for 1, 267 3, 5 and 7hr, and then processed for immunoblot analysis to detect PARP2 ( Fig. 2A) . 268
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