Author: Aaron S. Mendez; Carolin Vogt; Jens Bohne; Britt A. Glaunsinger
Title: Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX Document date: 2018_5_13
ID: 298cbr1x_16
Snippet: We next quantitatively measured the catalytic efficiency of SOX towards each of the above RNA substrates. Despite SOX having WT binding affinity for the predicted cleavage site mutant LIMD1 A-G, there was a 4-fold defect in its ability to degrade this mutant (Fig. 5C ). Even more marked defects in SOX catalytic efficiency were observed for the binding site mutant LIMD1 3XA-G, the loop mutant LIMD1 zipper, and the pre-miRNA K2-31 (Fig. 5C ). Colle.....
Document: We next quantitatively measured the catalytic efficiency of SOX towards each of the above RNA substrates. Despite SOX having WT binding affinity for the predicted cleavage site mutant LIMD1 A-G, there was a 4-fold defect in its ability to degrade this mutant (Fig. 5C ). Even more marked defects in SOX catalytic efficiency were observed for the binding site mutant LIMD1 3XA-G, the loop mutant LIMD1 zipper, and the pre-miRNA K2-31 (Fig. 5C ). Collectively, these data indicate that efficient RNA cleavage requires both an appropriate SOX binding site and a suitable cut site. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/320929 doi: bioRxiv preprint
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