Author: Yong Zhang; Wanjun Zhao; Yonghong Mao; Shisheng Wang; Yi Zhong; Tao Su; Meng Gong; Xiaofeng Lu; Jingqiu Cheng; Hao Yang
Title: Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry Document date: 2020_3_29
ID: 8xck5832_5
Snippet: Intact N-glycopeptide analysis can provide N-glycoproteomic information, including the composition and number of N-glycans decorating a specific N-glycopeptide or N-glycosite. The potential N-glycopeptides in the S protein sequence are shown in Fig. S1 . A comparison of the intact N-glycopeptides' spectra to the total spectra showed that the average enrichment efficiency of the Zic-HILIC materials reached up to 97%......
Document: Intact N-glycopeptide analysis can provide N-glycoproteomic information, including the composition and number of N-glycans decorating a specific N-glycopeptide or N-glycosite. The potential N-glycopeptides in the S protein sequence are shown in Fig. S1 . A comparison of the intact N-glycopeptides' spectra to the total spectra showed that the average enrichment efficiency of the Zic-HILIC materials reached up to 97%.
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