Author: Lu Guo; Xuehan Sun; Xinge Wang; Chen Liang; Haiping Jiang; Qingqin Gao; Moyu Dai; Bin Qu; Sen Fang; Yihuan Mao; Yangcan Chen; Guihai Feng; Qi Gu; Liu Wang; Ruiqi Rachel Wang; Qi Zhou; Wei Li
Title: SARS-CoV-2 detection with CRISPR diagnostics Document date: 2020_4_11
ID: 2una9767_1
Snippet: The novel coronavirus (CoV) disease termed caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) is causing a massive pandemic worldwide, threatening public health systems across the globe. During this ongoing COVID-19 outbreak, nucleic acid detection has played an important role in early diagnosis. Here we report a SARS-CoV-2 detection protocol using a CRISPR-based CRISPR diagnostic platform -CDetection (Cas12b-mediated DNA dete.....
Document: The novel coronavirus (CoV) disease termed caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) is causing a massive pandemic worldwide, threatening public health systems across the globe. During this ongoing COVID-19 outbreak, nucleic acid detection has played an important role in early diagnosis. Here we report a SARS-CoV-2 detection protocol using a CRISPR-based CRISPR diagnostic platform -CDetection (Cas12b-mediated DNA detection). By combining sample treatment protocols and nucleic acid amplification methods with CDetection, we have established an integrated viral nucleic acid detection platform -CASdetec (CRISPR-assisted detection). The detection limit of CASdetec for SARS-CoV-2 pseudovirus is 1 × 10 4 copies/mL, with no cross reactivity observed. Our assay design and optimization process can provide guidance for future CRISPR-based nucleic acid detection assay development and optimization.
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