Selected article for: "genome sequence and high similarity"
Author: Lu Guo; Xuehan Sun; Xinge Wang; Chen Liang; Haiping Jiang; Qingqin Gao; Moyu Dai; Bin Qu; Sen Fang; Yihuan Mao; Yangcan Chen; Guihai Feng; Qi Gu; Liu Wang; Ruiqi Rachel Wang; Qi Zhou; Wei Li
Title: SARS-CoV-2 detection with CRISPR diagnostics
  • Document date: 2020_4_11
    • ID: 2una9767_6
    Snippet: According to the published SARS-CoV-2 whole genome sequence [8] , we designed 7 sgRNAs around the RdRp locus, as recommended by the World Health Organization (WHO) [2] (Supplementary information, Fig. S2a ). Due to its high similarity to SARS-CoV, we ran initial experiments on both SARS-CoV-2 and SARS-CoV plasmids. According to fluorescence kinetics studies, sgRNA-3 stood out in not only being able to distinguish between the 2 similar coronavirus.....
    Document: According to the published SARS-CoV-2 whole genome sequence [8] , we designed 7 sgRNAs around the RdRp locus, as recommended by the World Health Organization (WHO) [2] (Supplementary information, Fig. S2a ). Due to its high similarity to SARS-CoV, we ran initial experiments on both SARS-CoV-2 and SARS-CoV plasmids. According to fluorescence kinetics studies, sgRNA-3 stood out in not only being able to distinguish between the 2 similar coronaviruses but also being able to produce the most distinct As previously demonstrated, CRISPR is unable to detect any target DNA when there is less than 1-10 nM of amplification product within the reaction mix [7] . Hence, increasing the molecular collisions between CRISPR and target would be essential to improve sensitivity.

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