Author: Ke Wang; Wei Chen; Yu-Sen Zhou; Jian-Qi Lian; Zheng Zhang; Peng Du; Li Gong; Yang Zhang; Hong-Yong Cui; Jie-Jie Geng; Bin Wang; Xiu-Xuan Sun; Chun-Fu Wang; Xu Yang; Peng Lin; Yong-Qiang Deng; Ding Wei; Xiang-Min Yang; Yu-Meng Zhu; Kui Zhang; Zhao-Hui Zheng; Jin-Lin Miao; Ting Guo; Ying Shi; Jun Zhang; Ling Fu; Qing-Yi Wang; Huijie Bian; Ping Zhu; Zhi-Nan Chen
Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein Document date: 2020_3_14
ID: 5hpbjkft_29
Snippet: BioTek Instruments, Inc.). The ability of Meplazumab to compete with SP for CD147 binding was performed by competitive inhibition ELISA. The protein His-CD147 was coated onto the wells of microplate, the mixture of His-SP (RBD) and Meplazumab ( 2-fold dilution, 200-0.781 µg/mL) was added and incubated. Subsequently, the samples were incubated with anti-SARS-CoV-2 Spike antibody (40150-R007, Sino Biological, China) and then detected with HRP-labe.....
Document: BioTek Instruments, Inc.). The ability of Meplazumab to compete with SP for CD147 binding was performed by competitive inhibition ELISA. The protein His-CD147 was coated onto the wells of microplate, the mixture of His-SP (RBD) and Meplazumab ( 2-fold dilution, 200-0.781 µg/mL) was added and incubated. Subsequently, the samples were incubated with anti-SARS-CoV-2 Spike antibody (40150-R007, Sino Biological, China) and then detected with HRP-labeled goat anti-rabbit antibody for 1 hour at 37°C, the following steps were similar to those described earlier.
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