Author: Pongsiri, Piyathida; Praianantathavorn, Kesmanee; Theamboonlers, Apiradee; Payungporn, Sunchai; Poovorawan, Yong
Title: Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus Cord-id: u43ehmut Document date: 2012_5_31
ID: u43ehmut
Snippet: Abstract Objective To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. Methods We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. Results The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV
Document: Abstract Objective To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. Methods We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. Results The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. Conclusions This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
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