Author: Pongsiri, Piyathida; Praianantathavorn, Kesmanee; Theamboonlers, Apiradee; Payungporn, Sunchai; Poovorawan, Yong
Title: Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus Cord-id: u43ehmut Document date: 2012_5_31
ID: u43ehmut
Snippet: Abstract Objective To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. Methods We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. Results The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV
Document: Abstract Objective To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. Methods We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. Results The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. Conclusions This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
Search related documents:
Co phrase search for related documents- accession number and acute respiratory syndrome: 1, 2, 3
- accuracy specificity and acute phase: 1, 2
- accuracy specificity and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- acute respiratory syndrome and additionally cross: 1, 2, 3, 4
- acute respiratory syndrome and low remain: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
Co phrase search for related documents, hyperlinks ordered by date