iTRAQ-based proteomic and bioinformatic characterization of human mast cells upon infection by the influenza A virus strains H1N1 and H5N1.

Wu, Hongping; Zhang, Shouping; Huo, Caiyun; Zou, Shumei; Lian, Zhengxing; Hu, Yanxin

In our previous study, we demonstrated that mast cells can support replication of Influenza A virus (IAV), but how this occurs is poorly understood. Here, using quantitative mass spectrometry, we analyzed the proteome of human mast cells (HMCs) infected with different IAV strains at 12h post-infection (12 hpi). 41 differentially expressed proteins (DEPs) were identified in HMCs upon infection by the virulent H5N1 (A/Chicken/Henan/1/04) virus compared to the seasonal H1N1 (A/WSN/33) virus. Bioinformatic analyses confirmed that H1N1 significantly regulates the RNA degradation pathway via upregulation of CNOT4, while apoptosis could be suppressed by H5N1 through downregulation of the p53 signaling pathway with P≤0.05 at 12 hpi. The HIF-1 signaling pathway of HMCs is more susceptible to infection by H5N1 than by H1N1 virus. This article is protected by copyright. All rights reserved.