Selected article for: "quality control and single cell"
Author: Allen Wang; Joshua A Chiou; Olivier B Poirion; Justin Buchanan; Michael J Valdez; Jamie M Verheyden; Xiaomeng Hou; Minzhe Guo; Jacklyn M Newsome; Parul Kudtarkar; Dina A Faddah; Kai Zhang; Randee E Young; Justinn Barr; Ravi Misra; Heidie Huyck; Lisa Rogers; Cory Poole; Jeffery A Whitsett; Gloria Pryhuber; Yan Xu; Kyle J Gaulton; Sebastian Preissl; Xin Sun
Title: Single Nucleus Multiomic Profiling Reveals Age-Dynamic Regulation of Host Genes Associated with SARS-CoV-2 Infection
  • Document date: 2020_4_14
    • ID: 5kvq6jtx_5
    Snippet: To generate snRNA-seq libraries, we used the droplet-based Chromium Single Cell 175 3' solution (10x Genomics) (Zheng et al., 2017) . The datasets showed a clear separation 176 of nuclei from background in the knee plot ( Figure S1A ). The average number of nuclei 177 that passed initial quality control filtering per sample was 6,676 for 30wk GA , 7,379 for 3yo, 178 and 4,217 for 30yo ( Figure S1B ). Since we profiled nuclei with a high fraction .....
    Document: To generate snRNA-seq libraries, we used the droplet-based Chromium Single Cell 175 3' solution (10x Genomics) (Zheng et al., 2017) . The datasets showed a clear separation 176 of nuclei from background in the knee plot ( Figure S1A ). The average number of nuclei 177 that passed initial quality control filtering per sample was 6,676 for 30wk GA , 7,379 for 3yo, 178 and 4,217 for 30yo ( Figure S1B ). Since we profiled nuclei with a high fraction of nascent, 179 unspliced RNA molecules, sequencing reads were mapped to an exon+intron reference. 180

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