Selected article for: "control plasmid and IDT SARS control plasmid"
Author: Fuqing Wu; Amy Xiao; Jianbo Zhang; Xiaoqiong Gu; Wei Lin Lee; Kathryn Kauffman; William Hanage; Mariana Matus; Newsha Ghaeli; Noriko Endo; Claire Duvallet; Katya Moniz; Timothy Erickson; Peter Chai; Janelle Thompson; Eric Alm
Title: SARS-CoV-2 titers in wastewater are higher than expected from clinically confirmed cases
  • Document date: 2020_4_7
    • ID: atnxkvy6_20
    Snippet: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.05.20051540 doi: medRxiv preprint 12,000 X g for 2 hours or until a pellet was visible. The viral pellet was then resuspended in Trizol (Thermofisher) for RNA extraction, followed by reverse transcription (reverse transcriptase, NEB) and qPCR (TaqMan fast advanced master mix, Thermofisher) with CDC primers (IDT) for the nucleocapsid N gen.....
    Document: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.05.20051540 doi: medRxiv preprint 12,000 X g for 2 hours or until a pellet was visible. The viral pellet was then resuspended in Trizol (Thermofisher) for RNA extraction, followed by reverse transcription (reverse transcriptase, NEB) and qPCR (TaqMan fast advanced master mix, Thermofisher) with CDC primers (IDT) for the nucleocapsid N gene. Positive control (IDT) is a plasmid containing complete nucleocapsid gene from SARS-CoV-2, and used to create the standard curves for N1, N2, and N3 primers.

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