Author: Fuqing Wu; Amy Xiao; Jianbo Zhang; Xiaoqiong Gu; Wei Lin Lee; Kathryn Kauffman; William Hanage; Mariana Matus; Newsha Ghaeli; Noriko Endo; Claire Duvallet; Katya Moniz; Timothy Erickson; Peter Chai; Janelle Thompson; Eric Alm
Title: SARS-CoV-2 titers in wastewater are higher than expected from clinically confirmed cases Document date: 2020_4_7
ID: atnxkvy6_4
Snippet: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.05.20051540 doi: medRxiv preprint Initial testing with PCR using primers specific for the SARS-CoV-2 S gene (9) indicated that both samples collected from the treatment facility on March 18 had a positive signal for SARS-CoV-2. We confirmed the signal by identifying a PCR product at ~150 bp. A no-template control for the PCR assays was ne.....
Document: is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.05.20051540 doi: medRxiv preprint Initial testing with PCR using primers specific for the SARS-CoV-2 S gene (9) indicated that both samples collected from the treatment facility on March 18 had a positive signal for SARS-CoV-2. We confirmed the signal by identifying a PCR product at ~150 bp. A no-template control for the PCR assays was negative. Sanger sequencing of the PCR products confirmed a 97-98% identity match to the SARS-CoV-2 S gene ( Figure 1 ).
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