Selected article for: "active site and RNA synthesis"
Author: Anniina Vihervaara; Dig Bijay Mahat; Samu V. Himanen; Malin A.H. Blom; John T. Lis; Lea Sistonen
Title: Stress-Induced Transcriptional Memory Accelerates Promoter-Proximal Pause-Release and Decelerates Termination over Mitotic Divisions
  • Document date: 2019_3_14
    • ID: i3owd6b0_10
    Snippet: In this study, we used heat shock to provoke a genome-wide change in gene and enhancer 80! transcription, and asked whether proteotoxic stress reprograms nascent transcription and 81! transcriptional responsiveness over mitotic divisions. To map RNA synthesis at nucleotide 82! resolution, we used Precision Run-On sequencing (PRO-seq) that tracks incorporation of a single 83! biotinylated nucleotide at the active site of each nascent transcript (K.....
    Document: In this study, we used heat shock to provoke a genome-wide change in gene and enhancer 80! transcription, and asked whether proteotoxic stress reprograms nascent transcription and 81! transcriptional responsiveness over mitotic divisions. To map RNA synthesis at nucleotide 82! resolution, we used Precision Run-On sequencing (PRO-seq) that tracks incorporation of a single 83! biotinylated nucleotide at the active site of each nascent transcript (Kwak et al., 2013) .

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