Author: Yasunori Watanabe; Joel D. Allen; Daniel Wrapp; Jason S. McLellan; Max Crispin
Title: Site-specific analysis of the SARS-CoV-2 glycan shield Document date: 2020_3_28
ID: 63j4qc7d_24
Snippet: Purified SARS-CoV-2 spike was diluted to a concentration of 0.04 mg/mL using 2 mM Tris pH 8.0, 200 mM NaCl and 0.02% NaN3 before being applied to a plasma cleaned CF400-Cu grid (Electron Microscopy Sciences). Protein was then stained using methylamine tungstate (Nanoprobes) before being allowed to dry at room temperature for 15 minutes. This grid was imaged in a Talos TEM (Thermo Fisher Scientific) equipped with a Ceta 16M detector......
Document: Purified SARS-CoV-2 spike was diluted to a concentration of 0.04 mg/mL using 2 mM Tris pH 8.0, 200 mM NaCl and 0.02% NaN3 before being applied to a plasma cleaned CF400-Cu grid (Electron Microscopy Sciences). Protein was then stained using methylamine tungstate (Nanoprobes) before being allowed to dry at room temperature for 15 minutes. This grid was imaged in a Talos TEM (Thermo Fisher Scientific) equipped with a Ceta 16M detector.
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