Author: Natalia B. Hubbs; Mareena M. Whisby-Pitts; Jonathan L. McMurry
Title: Kinetic Analysis of Bacteriophage Sf6 Binding to Outer Membrane Protein A Using Whole Virions Document date: 2019_1_2
ID: ktds6nla_10
Snippet: Media and strains. Bacterial growth, plating experiments, and preparations of Sf6 phage stocks were all completed in Lysogeny broth (LB). Bacteriophage Sf6 (clear plaque mutant [33] ) was propagated on ompA -C -S. flexneri (dual ompA and ompC gene knock out), as previously described [25] . Phage used for in vitro genome ejection experiments were stored in phage buffer (10 mM Tris, pH 7.6 and 10 mM MgCl 2 ) and phage used for BLI experiments were .....
Document: Media and strains. Bacterial growth, plating experiments, and preparations of Sf6 phage stocks were all completed in Lysogeny broth (LB). Bacteriophage Sf6 (clear plaque mutant [33] ) was propagated on ompA -C -S. flexneri (dual ompA and ompC gene knock out), as previously described [25] . Phage used for in vitro genome ejection experiments were stored in phage buffer (10 mM Tris, pH 7.6 and 10 mM MgCl 2 ) and phage used for BLI experiments were stored in NaOAc buffer (10 mM sodium acetate, pH 4.0 and 2 mM MgCl 2 ). S. flexneri strain PE577 was used for phage plating experiments [22] . OmpA-TM proteins ("TM" = transmembrane portion of OmpA that lacks the periplasmic domain, and has been shown to be sufficient to induce genome All rights reserved. No reuse allowed without permission.
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