Author: Wanchao Yin; Chunyou Mao; Xiaodong Luan; Dan-Dan Shen; Qingya Shen; Haixia Su; Xiaoxi Wang; Fulai Zhou; Wenfeng Zhao; Minqi Gao; Shenghai Chang; Yuan-Chao Xie; Guanghui Tian; He-Wei Jiang; Sheng-Ce Tao; Jingshan Shen; Yi Jiang; Hualiang Jiang; Yechun Xu; Shuyang Zhang; Yan Zhang; H. Eric Xu
Title: Structural Basis for the Inhibition of the RNA-Dependent RNA Polymerase from SARS-CoV-2 by Remdesivir Document date: 2020_4_9
ID: 7v7pzclb_29
Snippet: The following reference-free 2D classification in cryoSPARC was performed and produced 2,160,423 particle projections for two rounds of Hetero Refinement. One selected subset containing 257,941 projections was used to obtain a map with resolution of 3.2 Ã…. The selected subset of particle projections was subsequently imported back to RELION-3.0-beta2 and subjected to consecutive rounds of 3D refinement and Bayesian polishing. Another round of 3D .....
Document: The following reference-free 2D classification in cryoSPARC was performed and produced 2,160,423 particle projections for two rounds of Hetero Refinement. One selected subset containing 257,941 projections was used to obtain a map with resolution of 3.2 Ã…. The selected subset of particle projections was subsequently imported back to RELION-3.0-beta2 and subjected to consecutive rounds of 3D refinement and Bayesian polishing. Another round of 3D classification with mask on the complex produced a subset of 81,494 particle projections for the final reconstruction. The final map has an indicated global resolution of 2.8Ã… at a Fourier shell correlation (FSC) of 0.143.
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