Author: Dhurvas Chandrasekaran Dinesh; Dominika Chalupska; Jan Silhan; Vaclav Veverka; Evzen Boura
Title: Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein Document date: 2020_4_5
ID: ezpjdz55_16
Snippet: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022194 doi: bioRxiv preprint equipped with a triple-resonance ( 15 N/ 13 C/ 1 H) cryoprobe. The sample volume was either 0.16 or 0.35 mL, in SEC buffer, 5% D2O/90-95% H2O. A series of double-and triple-resonance spectra (7, 8) were recorded to obtain se.....
Document: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022194 doi: bioRxiv preprint equipped with a triple-resonance ( 15 N/ 13 C/ 1 H) cryoprobe. The sample volume was either 0.16 or 0.35 mL, in SEC buffer, 5% D2O/90-95% H2O. A series of double-and triple-resonance spectra (7, 8) were recorded to obtain sequence-specific resonance assignment. We used I-PINE assignment tool (9) implemented in NMRFAM-SPARKY (10) for initial automatic assignment. 1 H-1 H distance restraints were derived from 3D 15 N/ 1 H NOESY-HSQC and 13 C/ 1 H NOESY-HMQC, which were acquired using a NOE mixing time of 100 ms.
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