Author: Cláudia Pereira; Rita M. Reis; José B. Gama; Dhanya K. Cheerambathur; Ana X. Carvalho; Reto Gassmann
Title: Self-assembly of the RZZ complex into filaments drives kinetochore expansion in the absence of microtubule attachment Document date: 2018_3_15
ID: ajkhpw5f_42
Snippet: For production of double stranded RNA (dsRNA ; Table S3) The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/282707 doi: bioRxiv preprint Immunofluorescence in C. elegans embryos was carried out as described [47] , using the following primary antibodies: goat anti-GFP, 1:15000; rabbit anti-mCherry OD78, 1 µg/mL; mouse anti-aï¡-Tubulin DM1a, 1:1000 (Sigma-Aldrich); mouse anti-FL.....
Document: For production of double stranded RNA (dsRNA ; Table S3) The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/282707 doi: bioRxiv preprint Immunofluorescence in C. elegans embryos was carried out as described [47] , using the following primary antibodies: goat anti-GFP, 1:15000; rabbit anti-mCherry OD78, 1 µg/mL; mouse anti-aï¡-Tubulin DM1a, 1:1000 (Sigma-Aldrich); mouse anti-FLAG M2, 1:1000 (Sigma-Aldrich); rabbit anti-KNL-1 OD33, 1 µg/mL; rabbit anti-BUB-1 OD31, 1 µg/mL; rabbit anti-ZWL-1 OD85, 1 µg/mL; rabbit anti-SPDL-1 OD164, 1:7000. Antibodies OD31, OD33, OD78, OD85, and OD164 were a gift from Arshad Desai.
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