Selected article for: "binding domain and human cell"
Author: Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia
Title: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
  • Document date: 2020_4_9
    • ID: fvjh3g7v_8
    Snippet: It was reported that a pseudotyped VSV and retrovirus bearing a SARS-CoV-S protein variant with a truncation in the cytoplasmic tail had much higher infection efficiency than that with full-length S protein [11, 14] . In this study, the VSVdG pseudotyped with full-length SARS-CoV-2-S protein or truncated SARS-CoV-2-Sdel18 protein with C-terminal 18 aa truncation were compared, we found that the infection efficiency of VSV-SARS-CoV-2-Sdel18 was mu.....
    Document: It was reported that a pseudotyped VSV and retrovirus bearing a SARS-CoV-S protein variant with a truncation in the cytoplasmic tail had much higher infection efficiency than that with full-length S protein [11, 14] . In this study, the VSVdG pseudotyped with full-length SARS-CoV-2-S protein or truncated SARS-CoV-2-Sdel18 protein with C-terminal 18 aa truncation were compared, we found that the infection efficiency of VSV-SARS-CoV-2-Sdel18 was much higher than VSV-SARS-CoV-2-S. Among the cell lines used in this study, BHK21-hACE2 stably expressing human ACE2 was most sensitive for infection, while Vero-E6 was most suitable for virus package. A neutralization assay for antibody screening and validation was established based on VSV-SARS-CoV-2-Sdel18 and BHK21-hACE2 cell, and 7 strains of neutralizing monoclonal antibodies targeting receptor binding domain (RBD) of SARS-CoV-2-S were obtained. Most importantly, the correlation between pseudovirus system and live virus system was verified. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.026948 doi: bioRxiv preprint Han) cells were maintained in high glucose DMEM (SIGMA-ALDRICH) supplemented with 10% FBS (GIBCO), penicillin (100 IU/mL), streptomycin (100 μg/mL) in a 5% CO2 environment at 37°C and passaged every 2 days. BHK21-

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