Selected article for: "active site and HCV polymerase"
Author: Yan Gao; Liming Yan; Yucen Huang; Fengjiang Liu; Yao Zhao; Lin Cao; Tao Wang; Qianqian Sun; Zhenhua Ming; Lianqi Zhang; Ji Ge; Litao Zheng; Ying Zhang; Haofeng Wang; Yan Zhu; Chen Zhu; Tianyu Hu; Tian Hua; Bing Zhang; Xiuna Yang; Jun Li; Haitao Yang; Zhijie Liu; Wenqing Xu; Luke W. Guddat; Quan Wang; Zhiyong Lou; Zihe Rao
Title: Structure of RNA-dependent RNA polymerase from 2019-nCoV, a major antiviral drug target
  • Document date: 2020_3_17
    • ID: glfxrla9_8
    Snippet: The polymerase domain adopts the conserved architecture of the viral polymerase family (11) and is composed of three subdomains, including a fingers subdomain (residues S397-A581 and K621-G679), a palm subdomain (residues T582-P620 and T680-Q815), and a thumb subdomain (residues H816-E920) (Fig. 1) . The catalytic cations, which are usually observed in viral polymerase structure for RNA synthesis (12, 13) are not observed, possibly because mangan.....
    Document: The polymerase domain adopts the conserved architecture of the viral polymerase family (11) and is composed of three subdomains, including a fingers subdomain (residues S397-A581 and K621-G679), a palm subdomain (residues T582-P620 and T680-Q815), and a thumb subdomain (residues H816-E920) (Fig. 1) . The catalytic cations, which are usually observed in viral polymerase structure for RNA synthesis (12, 13) are not observed, possibly because manganese In this structure, the four positively charged solvent-accessible paths converge into a central cavity 30 where the RdRp motifs mediate template-directed RNA synthesis (Fig. 3D ). The configurations of the template/primer entry paths, the nucleoside triphosphate (NTP) entry channel, and the nascent strand exit path are similar to those described for HCV and PV polymerase (14) (Figs. 3, B and C). The NTP entry channel is formed by a set of hydrophilic residues, including K545, R553 and R555 in motif F. The RNA template is expected to enter the active site composed of motifs A and 35 C through a groove clamped by motif F and G. Motif E and the thumb subdomain support the primer strand. The product-template hybrid exits the active site through the RNA exit tunnel at the front side of the polymerase.

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