Stress-induced phospho-ubiquitin formation causes parkin degradation

Lyudmila Kovalchuke; Eugene V. Mosharov; Oren A. Levy; Lloyd A. Greene

. CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484857 doi: bioRxiv preprint L-DOPA-induced oxidative stress contributes to parkin loss Given that L-DOPA has been shown to cause oxidative stress in cultured cells [56] , [60] , [61] , we examined whether L-DOPA-induced oxidative stress plays a role in parkin loss. To do this, we treated differentiated PC12 cells with L-DOPA in the presence of the antioxidant glutathione. Glutathione (GSH) significantly attenuated parkin loss from L-DOPA, but this rescue was not complete (43.3 ± 3.9% loss with L-DOPA alone vs. 18.9 ± 6.5% loss with L-DOPA + GSH, N = 3, p = 0.03; p = 0.03 for L-DOPA + GSH vs. GSH alone, N = 3) ( Fig. 2A) .