Selected article for: "attenuator hairpin and hairpin sequence"

Author: Jamie A. Kelly; Jonathan D. Dinman
Title: Structural and functional conservation of the programmed -1 ribosomal frameshift signal of SARS-CoV-2
  • Document date: 2020_3_15
  • ID: 62izhj8d_10
    Snippet: The structure of the SARS-CoV -1 PRF signal was previously determined to include a threestemmed mRNA pseudoknot (11) . Using this as a guide, the single C to A base difference between the core SARS-CoV and SARS-CoV-2 -1 PRF signals maps to a loop that is not predicted to alter the structure of the -1 PRF stimulating element (7) (Fig. 1C) . In contrast, the attenuator hairpin contains six differences in the nucleotide sequence between the two viru.....
    Document: The structure of the SARS-CoV -1 PRF signal was previously determined to include a threestemmed mRNA pseudoknot (11) . Using this as a guide, the single C to A base difference between the core SARS-CoV and SARS-CoV-2 -1 PRF signals maps to a loop that is not predicted to alter the structure of the -1 PRF stimulating element (7) (Fig. 1C) . In contrast, the attenuator hairpin contains six differences in the nucleotide sequence between the two viruses, and the SARS-CoV-2 element is predicted to be less stable than its SARS-CoV counterpart (Fig. 1D) . This suggests that the attenuation activity of this element is not as important as the ability to direct -1 PRF per se.

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