Selected article for: "ELISA detect and immunosorbent assay"

Author: Li, Lin-hai; Shi, Yu-ling; Li, Ping; Xu, De-xing; Wan, Gen-ping; Gu, Xiao-qiong; Zhang, Xiao-ling; Ma, Qing-jun; Cao, Cheng
Title: [Detection and analysis of SARS coronavirus-specific antibodies in sera from non-SARS children].
  • Cord-id: w0ceb715
  • Document date: 2003_1_1
  • ID: w0ceb715
    Snippet: OBJECTIVE To examine the presence of severe acute respiratory syndrome (SARS) coronavirus-specific antibodies in the sera from non-SARS children. METHODS Indirect immunofluorescent assay and double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect the virus-specific antibodies in sera of 1,060 non-SARS children in Guangzhou. RESULTS All the serum samples from the 1,060 non-SARS children were negative for both IgG and IgM antibodies against SARS coronavirus as determi
    Document: OBJECTIVE To examine the presence of severe acute respiratory syndrome (SARS) coronavirus-specific antibodies in the sera from non-SARS children. METHODS Indirect immunofluorescent assay and double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect the virus-specific antibodies in sera of 1,060 non-SARS children in Guangzhou. RESULTS All the serum samples from the 1,060 non-SARS children were negative for both IgG and IgM antibodies against SARS coronavirus as determined by indirect immunofluorescent assay, with only two serum samples showing weak positivity for SARS coronavirus-specific antibodies identified by double-antigen sandwich ELISA. CONCLUSION No SARS coronavirus-specific antibody are present in the sera of non-SARS children.

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