Structural basis to design multi-epitope vaccines against Novel Coronavirus 19 (COVID19) infection, the ongoing pandemic emergency: an in silico approach

Sukrit Srivastava; Sonia Verma; Mohit Kamthania; Rupinder Kaur; Ruchi Kiran Badyal; Ajay Kumar Saxena; Ho-Joon Shin; Kailash Pandey; Michael Kolbe

Protrusion Index (PI) of 0.9; and so on. The discontinuous epitopes predicted by 543 the ElliPro tool are clustered based on the distance "R" in Ã… between two 544 residue's centers of mass lying outside of the largest possible ellipsoid. The 545 larger value of R indicates larger distant residues (residue discontinuity) are 546 Screening of Helper T lymphocyte (HTL) epitopes. The screening of helper T 626 lymphocyte (HTL) epitopes from eleven different proteins of SARS-CoV-2 was 627 performed based on "Percentile rank". The smaller the value of percentile rank 628 the higher would be the affinity of the peptide with its respective HLA allele 629 binders. The 33 epitopes with high percentile ranking were shortlisted ( Omega. The analysis has shown that several epitopes of CTL, HTL and B cell 728 were having amino acid sequences overlap. The CTL, HTL and B cell epitopes 729 having two or more than two amino acid residues overlap are shown in Fig.2 . The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.01.019299 doi: bioRxiv preprint