Author: Ramasubramanian Sundaramoorthy; Amanda L. Hughes; Hassane El-Mkami; David Norman; Tom Owen-Hughes
Title: Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome Document date: 2018_3_30
ID: ct2zhauz_37
Snippet: We thank Daniel Claire electron Bio-Imaging Centre (eBIC), Diamond light source Ltd, UK for data collection with respect to the 4.5 Ã… structure. We The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/290874 doi: bioRxiv preprint C in Auto-induction media and the purification of the protein was carried out typically as described in Ryan et al. After the purification of the protein the GST tag was .....
Document: We thank Daniel Claire electron Bio-Imaging Centre (eBIC), Diamond light source Ltd, UK for data collection with respect to the 4.5 Ã… structure. We The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/290874 doi: bioRxiv preprint C in Auto-induction media and the purification of the protein was carried out typically as described in Ryan et al. After the purification of the protein the GST tag was cleaved with precision protease and the tag cleaved proteins were subjected to size exclusion chromatography using Superdex S200 10/300 GL columns (GE Healthcare). Expression and purification of Xenopus laevis histones were carried out as described previously (Luger et al., 1999) .
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