Association analysis of vitamin D receptor gene polymorphisms in North England population with Type 2 diabetes mellitus.

Sattar, Naila Abdul; Shaheen, Sumera; Hussain, Fatma; Jamil, Amer

Background Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens. Aim The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM). Five hundred T2DM patients and 200 healthy subjects with normal HbA1c (≤ 5.0 %), fasting blood sugar (≤ 120 mg/dL) and random blood sugar (≤ 140 mg/dL) were enrolled. Metholodgy The genotypes were found by polymerase chain reaction restriction fragment length polymorphism and DNA sequencing. Results revealed that no considerable differences in frequencies of genotype and allele of the Bsm I and Fok I polymorphisms between healthy and patients in the North England (For Fok I: OR = 1.11, 95% CI: 0.72-1.12; for Bsm I: OR = 1.35, 95% CI: 0.79-1.98). Conclusion It is recommended that both following polymorphisms of vitamin D receptor gene may not considerably add to the progression of T2DM in the North England.